INTEGRATION EFFICIENCY AND GENETIC RECOMBINATION IN PNEUMOCOCCAL TRANSFORMATION
نویسندگان
چکیده
منابع مشابه
On the Role of Integrity of Dna Particles in Genetic Recombination during Pneumococcal Transformation.
8 The term "freezing of the particles" is used for convenience. As it is uncertain whether or not there is an alteration in the physical state of the particles when frozen at -20'C, the term is therefore descriptive of the state of the suspending medium. 9 Weber, M. M., and G. Rosso, Fed. Proc., 22, 526 (1963). lo Gale, P. H., B. H. Arison, N. R. Trenner, A. C. Page, Jr., K. Folkers, and A. F. ...
متن کاملConversion of deletions during recombination in pneumococcal transformation.
Genetic analysis of 16 deletions obtained in the amiA locus of pneumococcus is described. When present on donor DNA, all deletions increased drastically the frequency of wild-type recombinants in two-point crosses. This effect was maximal for deletions longer than 200 bases. It was reduced for heterologies shorter than 76 bases and did not exist for very short deletions. In three-point crosses ...
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HE science of genetics has undergone a tremendous development as a result T of the discovery of novel systems of exchange of genetic material. The first new system to be discovered was that of DNA-induced bacterial transformation (AVERY, MACLEOD and MCCARTY 1944) , yet as a purely genetic tool this system has been little used, in spite of possessing unique experimental possibilities. The presen...
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Protoplasts were produced from the thermophilic actinomycete Thermomonospora fusca and were regenerated to 0.1% of the direct count on regeneration agar. Recombination after protoplast fusion was demonstrated with drug-resistant mutants of T. fusca YX. A single thiostrepton-resistant colony was isolated after transformation of T. fusca YX with the streptomycete vector pIJ702, providing the firs...
متن کاملGenetic Studies of Recombining DNA in Pneumococcal Transformation
The results of genetic fine structure experiments, performed on the amiA locus of Pneumococcus are summarized. The peculiar feature of transformation genetics is that a given donor marker mutation transforms with an efficiency characteristic of the mutated site. In spite of this difficulty, mapping procedures have been devised and quantitative recombination studies performed. It is concluded fr...
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ژورنال
عنوان ژورنال: Genetics
سال: 1966
ISSN: 1943-2631
DOI: 10.1093/genetics/53.1.207